·    Assess family history: looking at age and weight related issues of individual and family members

·    Other pituitary deficits with normal IGF

·    Insulin (0.1–0.15 IU/kg) intravenously sufficient to cause adequate hypoglycemia (<40mg/dL) (<2.2 nmol/L)

·    Blood samples are collected for measurement of serum cortisol at –15, 0, 30, 45, 60 and 90 min

Metyrapone test

·    30 mg/kg orally at midnight with a snack to minimize gastrointestinal discomfort

·    Blood for serum 11b-deoxycortisol, ACTH and cortisol are obtained at 8 AM

·    100 µg recombinant human CRH is given intravenously

·    Blood samples for serum cortisol are collected at –15, 0, 30, 45 and 60 min

·    250 µg recombinant human ACTH and serum cortisol, given intravenously

·    Responses are assessed at 0, 30 and 60 min